Thursday, August 14 Thursday This week we reveal the resulting gel electrophoresis conducted last week. First we fix it with ethanol (300ml), acetic acid (100ml) and water (to fill a liter), leaving the gel for 1 hour. The dyed with silver nitrate (0.5 g per 0.5 l of solution prepared) for 1 hour. Eventually reveal. To this, add a solution of 21.6 ml of formaldehyde (0.4%), 30g NaOH (1.5%) and free water (up to complete the 2l). After this step we see the bands obtained.
Thursday July 24 July 17 July 10 This week we electrophoresis of DNAs obtained from the PCR to last week. We prepare Cuba's 20 x 40, the gel (acrylamide, ethylene glycol, tristaurina EDTA buffer, free water, ammonium persulfate and fear), then sow the samples (DNAs results of the 3 types of different DNA extraction, 2 normal heteroduplex between patient and normal) and let it run 24 hours. Next week I will reveal and analyze the results.
Thursday July 3 Thursday, 26 June This week we returned to extract DNA and test the "Mini-cards" that was left to Adrian last week to try. The mini-cards are some cards to store blood samples without freezing in pipes. For this, a few microliters of blood placed within each circle drawn on the board and allowed to dry for a while. There are different sizes of cards and each requires a different time to dry. Furthermore, some have only one large circle, while others have four circles, so you can keep blood from different patients. They also have a space for labeling. Next week we will make a PCR using blood stored in this way. Today also saw the differences in DNA sequencing according to the amount of magnesium is placed in the PCR. today Photos: http://www.slide.com/r/Xs6wkw4P1T8dyXDFTUeqN2KbDWnu35xs?previous_view=mscd_embedded_url&view=original
