PCR made at this meeting, which aims to amplify with specific primers a DNA fragment of interest.
to the mix that we add to the DNA samples we use water, buffer, dNTP, Mg, primers and TaqPol.
must meet certain conditions:
• The first should be 18 to 24 pairs of bases and can not have Police, polygamists, poly or political.
• The temperature of annealing (hybridization) can not be less than 50 º C and should be about 5 ° C below the melting temperature.
· T. Melting = 4 (G + C) + 2 (A + T). Should be given between 40 and 60%.
also did DNA extractions.
today Photos: http://www.slide.com/r/hC4UVSGL0j-zkSzfLnulSrSSEI9Iar8N?previous_view=mscd_embedded_url&view=original
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